The Animal Health Laboratory provides a front-line
service for the isolation and culture of salmonid viruses enzootic to Tasmania.
Where there is a suspicion of a new or exotic virus, AHL will refer samples for
testing to the Australian Centre for Disease Preparedness (ACDP), CSIRO, Geelong.
Cell lines available
Isolation of viruses can be undertaken on several different fish cell lines. The number and type of cell
lines used is dependent on the type of investigation required.
Before submitting
samples, please contact Fish Virology to discuss the most appropriate cell
lines to be used.
Virus isolation
Currently, salmonid viruses, which can be routinely
isolated and identified include:
- Tasmanian aquabirnavirus
- Tasmanian
aquareovirus
- pilchard orthomyxovirus
Sample submission
Book with the Animal Health Laboratory before sending in samples for virus culture.
We can advise on the
type and number of cell lines which should be used for the investigation, and
the most appropriate means of sample collection and submission.
Although it
may take up to seven days for the cell lines to be made ready, samples can be
submitted immediately after booking them in and will be held by the laboratory
until they can be inoculated on to the cell lines.
Submission can either be as whole-fish on ice or as
tissues collected at a field station or base laboratory.
General guidance on
sample collection can be found in the guidelines and procedure manual for Field Sampling of Fish for Disease Investigation and Health Monitoring.
Specific information
on collecting virology samples can be found in AHL LabFact Sampling for Virology Testing.
Reporting
Growth of salmonid viruses is slow. At minimum it will
take at least three weeks for viruses to appear and be identified. AHL will
provide interim results as appropriate.
Pre-smolt Atlantic salmon in a hatchery tank
Copyright: Scott Godwin